rabbit anti bdnf (Novus Biologicals)
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Rabbit Anti Bdnf, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+bdnf/BDNF+Antibody+(010)/us12553882-163-150-153
Average 94 stars, based on 19 article reviews
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Membrane:Article Title: Activation of Three Major Signaling Pathways After Endurance Training and Spinal Cord Injury Article Snippet: .. Subsequently, the membrane was blocked for 1.5 h in 5% milk in TBS-T. After blocking, the membrane was incubated overnight (4 °C) with the following primary antibodies: Article Title: Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys Article Snippet: A total of 60 μg protein per sample was resolved by SDS-PAGE on a 12% polyacrylamide gel and the protein bands were transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 10% milk for 1 h, the membrane was incubated with the following primary antibodies: rabbit anti-β-actin (1:10000, A5060; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-NeuN (1:1000, MAB377; Millipore), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology), rabbit anti-p-Erk (1:1000, 9101S; Cell Signaling Technology), rabbit anti-Erk (1:1000, 4695S; Cell Signaling Technology), rabbit anti-p-S6K (1:1000, PA5-17884; ThermoFisher Scientific, Schwerte, Germany), rabbit anti-S6K (1:1000, 2708S; Cell Signaling Technology), rabbit anti-light chain 3 (LC3) (1:1000, L7543; Sigma–Aldrich), mouse anti-P62 (1:1000, ab56416; Abcam, Cambridge, MA, USA), Blocking Assay:Article Title: Activation of Three Major Signaling Pathways After Endurance Training and Spinal Cord Injury Article Snippet: .. Subsequently, the membrane was blocked for 1.5 h in 5% milk in TBS-T. After blocking, the membrane was incubated overnight (4 °C) with the following primary antibodies: Article Title: Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys Article Snippet: A total of 60 μg protein per sample was resolved by SDS-PAGE on a 12% polyacrylamide gel and the protein bands were transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 10% milk for 1 h, the membrane was incubated with the following primary antibodies: rabbit anti-β-actin (1:10000, A5060; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-NeuN (1:1000, MAB377; Millipore), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology), rabbit anti-p-Erk (1:1000, 9101S; Cell Signaling Technology), rabbit anti-Erk (1:1000, 4695S; Cell Signaling Technology), rabbit anti-p-S6K (1:1000, PA5-17884; ThermoFisher Scientific, Schwerte, Germany), rabbit anti-S6K (1:1000, 2708S; Cell Signaling Technology), rabbit anti-light chain 3 (LC3) (1:1000, L7543; Sigma–Aldrich), mouse anti-P62 (1:1000, ab56416; Abcam, Cambridge, MA, USA), Incubation:Article Title: Activation of Three Major Signaling Pathways After Endurance Training and Spinal Cord Injury Article Snippet: .. Subsequently, the membrane was blocked for 1.5 h in 5% milk in TBS-T. After blocking, the membrane was incubated overnight (4 °C) with the following primary antibodies: Article Title: Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys Article Snippet: A total of 60 μg protein per sample was resolved by SDS-PAGE on a 12% polyacrylamide gel and the protein bands were transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 10% milk for 1 h, the membrane was incubated with the following primary antibodies: rabbit anti-β-actin (1:10000, A5060; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-NeuN (1:1000, MAB377; Millipore), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology), rabbit anti-p-Erk (1:1000, 9101S; Cell Signaling Technology), rabbit anti-Erk (1:1000, 4695S; Cell Signaling Technology), rabbit anti-p-S6K (1:1000, PA5-17884; ThermoFisher Scientific, Schwerte, Germany), rabbit anti-S6K (1:1000, 2708S; Cell Signaling Technology), rabbit anti-light chain 3 (LC3) (1:1000, L7543; Sigma–Aldrich), mouse anti-P62 (1:1000, ab56416; Abcam, Cambridge, MA, USA), Article Title: Physical exercise ameliorates the reduction of neural stem cell, cell proliferation and neuroblast differentiation in senescent mice induced by D-galactose. Article Snippet: .. Then, they were incubated with diluted chicken anti-nestin antibody (1:250; Novus, Littleton, CO, USA), rabbit antiKi67 antibody (1:1,000; Abcam, Cambridge, UK), goat anti-DCX antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Control:Article Title: Use of PAK4 and CRTC1 for treatment or diagnosis of brain degenerative disease Article Snippet: .. TABLE 1 Sample groups used in the experiments Age, years PMI, hours Group Gender (range) (range) YC 4M 41.9 39.1 (39-43) (31-51) AC 4M/3F 72.2 28.7 (63-79) (19-54) PD 5M/2F 71.9 39.2 (63-80) (20-65) PMI, post-mortem interval; YC, young control; AC, age-matched control; PD, Parkinson's disease; M, male; F, female. (1) Antibodies and Inhibitor Reagents As for the primary antibodies used in the experiments of the present invention, the following antibodies were used: rabbit anti-tyrosine hydroxylase (TH) (Pel-Freez, Brown Deer), mouse anti-TH (MAB5280, Millipore), rabbit anti-phospho-PAK4 (Ser474) (#3241, Cell Signaling), rabbit anti-PAK4 (ab19007, Abcam), rabbit anti-PAK5 (Abcam, ab11069), rabbit anti-PAK6 (Boster Biological Technology, PA1729), mouse anti-α-synsynsyn. (SNCA) (MAB5383, Abnova), rabbit anti-GAPDH (sc25778, Santa Cruz Biotechnology), mouse anti-Bcl-2 (sc7382, Santa Cruz Biotechnology), goat anti-allograft inflammatory factor 1 (Iba-1) (ab5076, Abcam), mouse anti-CD11b (MCA275C, Serotec, Oxford, UK), mouse anti-GFAP (Millipore), rabbit anti-GFP (sc8334, Santa Cruz Biotechnology), rabbit anti-Myc (sc789, Santa Cruz Biotechnology), |
