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rabbit anti bdnf  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rabbit anti bdnf
    Rabbit Anti Bdnf, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+bdnf/BDNF+Antibody+(010)/us12553882-163-150-153
    Average 94 stars, based on 19 article reviews
    rabbit anti bdnf - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Membrane:

    Article Title: Activation of Three Major Signaling Pathways After Endurance Training and Spinal Cord Injury
    Article Snippet: .. Subsequently, the membrane was blocked for 1.5 h in 5% milk in TBS-T. After blocking, the membrane was incubated overnight (4 °C) with the following primary antibodies: rabbit anti-BDNF (1:1000; Novus Biological, USA), rabbit anti-GDNF (1:500; abcam, UK), rabbit anti-TrkB (1:1000; Millipore, Germany), rabbit anti-PI3k (1:500; Cell Signaling, USA), rabbit anti-Akt (1:500; Cell Signaling, USA), rabbit anti-PLCγ (1:500; Cell Signaling, USA), rabbit anti-PKC (1:500; abcam, UK), rabbit anti-CAMKII (1:500; Cell Signaling, USA), rabbit anti-Ras (1:500; Cell Signaling, USA), rabbit anti-p44/42 MAPK (Erk1/2) (1:500; Cell Signaling, USA), rabbit anti-Rac1/Cdc42 (1:500; Cell Signaling, USA). .. Blots were washed with TBS-T (4 × 5 min) buffer and incubated with secondary antibody anti-rabbit HRP (1:5000; Santa Cruz Biotechnology, USA).

    Article Title: Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys
    Article Snippet: A total of 60 μg protein per sample was resolved by SDS-PAGE on a 12% polyacrylamide gel and the protein bands were transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 10% milk for 1 h, the membrane was incubated with the following primary antibodies: rabbit anti-β-actin (1:10000, A5060; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-NeuN (1:1000, MAB377; Millipore), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology), rabbit anti-p-Erk (1:1000, 9101S; Cell Signaling Technology), rabbit anti-Erk (1:1000, 4695S; Cell Signaling Technology), rabbit anti-p-S6K (1:1000, PA5-17884; ThermoFisher Scientific, Schwerte, Germany), rabbit anti-S6K (1:1000, 2708S; Cell Signaling Technology), rabbit anti-light chain 3 (LC3) (1:1000, L7543; Sigma–Aldrich), mouse anti-P62 (1:1000, ab56416; Abcam, Cambridge, MA, USA), rabbit anti-BDNF (1:1000, NBP1-59304; Novus Biologicals, Littleton, CO, USA), mouse anti-TrkB (1:500, NPB1-47898; Novus Biologicals), rabbit anti-cleaved caspase-3 (1:1000, 9661; Cell Signaling Technology), rabbit anti-caspase-3 (1:1000, 9662; Cell Signaling Technology), rabbit anti-cleaved caspase-9 (1:1000, 9507; Cell Signaling Technology), and mouse anti-caspase-9 (1:1000, 9508; Cell Signaling Technology). .. Membranes were incubated with secondary antibody (1:10000, LI-COR, Lincoln, NE, USA) and protein bands were visualized by enhanced chemiluminescence and quantified using ImageJ software (US National Institutes of Health).

    Blocking Assay:

    Article Title: Activation of Three Major Signaling Pathways After Endurance Training and Spinal Cord Injury
    Article Snippet: .. Subsequently, the membrane was blocked for 1.5 h in 5% milk in TBS-T. After blocking, the membrane was incubated overnight (4 °C) with the following primary antibodies: rabbit anti-BDNF (1:1000; Novus Biological, USA), rabbit anti-GDNF (1:500; abcam, UK), rabbit anti-TrkB (1:1000; Millipore, Germany), rabbit anti-PI3k (1:500; Cell Signaling, USA), rabbit anti-Akt (1:500; Cell Signaling, USA), rabbit anti-PLCγ (1:500; Cell Signaling, USA), rabbit anti-PKC (1:500; abcam, UK), rabbit anti-CAMKII (1:500; Cell Signaling, USA), rabbit anti-Ras (1:500; Cell Signaling, USA), rabbit anti-p44/42 MAPK (Erk1/2) (1:500; Cell Signaling, USA), rabbit anti-Rac1/Cdc42 (1:500; Cell Signaling, USA). .. Blots were washed with TBS-T (4 × 5 min) buffer and incubated with secondary antibody anti-rabbit HRP (1:5000; Santa Cruz Biotechnology, USA).

    Article Title: Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys
    Article Snippet: A total of 60 μg protein per sample was resolved by SDS-PAGE on a 12% polyacrylamide gel and the protein bands were transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 10% milk for 1 h, the membrane was incubated with the following primary antibodies: rabbit anti-β-actin (1:10000, A5060; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-NeuN (1:1000, MAB377; Millipore), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology), rabbit anti-p-Erk (1:1000, 9101S; Cell Signaling Technology), rabbit anti-Erk (1:1000, 4695S; Cell Signaling Technology), rabbit anti-p-S6K (1:1000, PA5-17884; ThermoFisher Scientific, Schwerte, Germany), rabbit anti-S6K (1:1000, 2708S; Cell Signaling Technology), rabbit anti-light chain 3 (LC3) (1:1000, L7543; Sigma–Aldrich), mouse anti-P62 (1:1000, ab56416; Abcam, Cambridge, MA, USA), rabbit anti-BDNF (1:1000, NBP1-59304; Novus Biologicals, Littleton, CO, USA), mouse anti-TrkB (1:500, NPB1-47898; Novus Biologicals), rabbit anti-cleaved caspase-3 (1:1000, 9661; Cell Signaling Technology), rabbit anti-caspase-3 (1:1000, 9662; Cell Signaling Technology), rabbit anti-cleaved caspase-9 (1:1000, 9507; Cell Signaling Technology), and mouse anti-caspase-9 (1:1000, 9508; Cell Signaling Technology). .. Membranes were incubated with secondary antibody (1:10000, LI-COR, Lincoln, NE, USA) and protein bands were visualized by enhanced chemiluminescence and quantified using ImageJ software (US National Institutes of Health).

    Incubation:

    Article Title: Activation of Three Major Signaling Pathways After Endurance Training and Spinal Cord Injury
    Article Snippet: .. Subsequently, the membrane was blocked for 1.5 h in 5% milk in TBS-T. After blocking, the membrane was incubated overnight (4 °C) with the following primary antibodies: rabbit anti-BDNF (1:1000; Novus Biological, USA), rabbit anti-GDNF (1:500; abcam, UK), rabbit anti-TrkB (1:1000; Millipore, Germany), rabbit anti-PI3k (1:500; Cell Signaling, USA), rabbit anti-Akt (1:500; Cell Signaling, USA), rabbit anti-PLCγ (1:500; Cell Signaling, USA), rabbit anti-PKC (1:500; abcam, UK), rabbit anti-CAMKII (1:500; Cell Signaling, USA), rabbit anti-Ras (1:500; Cell Signaling, USA), rabbit anti-p44/42 MAPK (Erk1/2) (1:500; Cell Signaling, USA), rabbit anti-Rac1/Cdc42 (1:500; Cell Signaling, USA). .. Blots were washed with TBS-T (4 × 5 min) buffer and incubated with secondary antibody anti-rabbit HRP (1:5000; Santa Cruz Biotechnology, USA).

    Article Title: Anterior thalamic nucleus stimulation protects hippocampal neurons by activating autophagy in epileptic monkeys
    Article Snippet: A total of 60 μg protein per sample was resolved by SDS-PAGE on a 12% polyacrylamide gel and the protein bands were transferred to a polyvinylidene difluoride membrane (Millipore, Billerica, MA, USA). .. After blocking with 10% milk for 1 h, the membrane was incubated with the following primary antibodies: rabbit anti-β-actin (1:10000, A5060; Sigma–Aldrich, St. Louis, MO, USA), mouse anti-NeuN (1:1000, MAB377; Millipore), rabbit anti-phosphorylated (p)-Akt (1:1000, 4060S; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-Akt (1:1000, 4691S; Cell Signaling Technology), rabbit anti-p-Erk (1:1000, 9101S; Cell Signaling Technology), rabbit anti-Erk (1:1000, 4695S; Cell Signaling Technology), rabbit anti-p-S6K (1:1000, PA5-17884; ThermoFisher Scientific, Schwerte, Germany), rabbit anti-S6K (1:1000, 2708S; Cell Signaling Technology), rabbit anti-light chain 3 (LC3) (1:1000, L7543; Sigma–Aldrich), mouse anti-P62 (1:1000, ab56416; Abcam, Cambridge, MA, USA), rabbit anti-BDNF (1:1000, NBP1-59304; Novus Biologicals, Littleton, CO, USA), mouse anti-TrkB (1:500, NPB1-47898; Novus Biologicals), rabbit anti-cleaved caspase-3 (1:1000, 9661; Cell Signaling Technology), rabbit anti-caspase-3 (1:1000, 9662; Cell Signaling Technology), rabbit anti-cleaved caspase-9 (1:1000, 9507; Cell Signaling Technology), and mouse anti-caspase-9 (1:1000, 9508; Cell Signaling Technology). .. Membranes were incubated with secondary antibody (1:10000, LI-COR, Lincoln, NE, USA) and protein bands were visualized by enhanced chemiluminescence and quantified using ImageJ software (US National Institutes of Health).

    Article Title: Physical exercise ameliorates the reduction of neural stem cell, cell proliferation and neuroblast differentiation in senescent mice induced by D-galactose.
    Article Snippet: .. Then, they were incubated with diluted chicken anti-nestin antibody (1:250; Novus, Littleton, CO, USA), rabbit antiKi67 antibody (1:1,000; Abcam, Cambridge, UK), goat anti-DCX antibody (1:50; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-BDNF (1:500; Novus, Littleton, CO, USA) or rabbit anti-pCREB (1:1,000; Millipore, Temecula, CA, USA) overnight, and subsequently exposed to biotinylated goat anti-chicken, rabbit anti-goat, or goat anti-rabbit IgG (diluted 1:200; Vector Labs., Burlingame, CA, USA) and streptavidin peroxidase complex (diluted 1:200, Vector Labs.). .. Then, the sections were visualized by reaction with 3,3’-diaminobenzidine tetrahydrochloride (Sigma, St. Louis, MO, USA).

    Control:

    Article Title: Use of PAK4 and CRTC1 for treatment or diagnosis of brain degenerative disease
    Article Snippet: .. TABLE 1 Sample groups used in the experiments Age, years PMI, hours Group Gender (range) (range) YC 4M 41.9 39.1 (39-43) (31-51) AC 4M/3F 72.2 28.7 (63-79) (19-54) PD 5M/2F 71.9 39.2 (63-80) (20-65) PMI, post-mortem interval; YC, young control; AC, age-matched control; PD, Parkinson's disease; M, male; F, female. (1) Antibodies and Inhibitor Reagents As for the primary antibodies used in the experiments of the present invention, the following antibodies were used: rabbit anti-tyrosine hydroxylase (TH) (Pel-Freez, Brown Deer), mouse anti-TH (MAB5280, Millipore), rabbit anti-phospho-PAK4 (Ser474) (#3241, Cell Signaling), rabbit anti-PAK4 (ab19007, Abcam), rabbit anti-PAK5 (Abcam, ab11069), rabbit anti-PAK6 (Boster Biological Technology, PA1729), mouse anti-α-synsynsyn. (SNCA) (MAB5383, Abnova), rabbit anti-GAPDH (sc25778, Santa Cruz Biotechnology), mouse anti-Bcl-2 (sc7382, Santa Cruz Biotechnology), goat anti-allograft inflammatory factor 1 (Iba-1) (ab5076, Abcam), mouse anti-CD11b (MCA275C, Serotec, Oxford, UK), mouse anti-GFAP (Millipore), rabbit anti-GFP (sc8334, Santa Cruz Biotechnology), rabbit anti-Myc (sc789, Santa Cruz Biotechnology), rabbit anti-BDNF (ERP1292, Novus Biologicals), rabbit anti-PGC-1α (NBP1-04676, Novus Biologicals), mouse anti-CRTC1 (ab176472, Abcam), rabbit anti-His (#2366S, Cell Signaling), GST-conjugated horseradish peroxidase (HRP) (ab3416, Abcam). ..



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    94
    Novus Biologicals rabbit anti bdnf
    (A) Representative images <t>showing</t> <t>GFP</t> and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, <t>AAV-DIO-BDNF-mCherry+sgRNA,</t> AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.
    Rabbit Anti Bdnf, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+bdnf/BDNF+Antibody+(010)/us12553882-163-150-153
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    96
    Cell Signaling Technology Inc rabbit polyclonal anti bdnf
    (A) Representative images <t>showing</t> <t>GFP</t> and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, <t>AAV-DIO-BDNF-mCherry+sgRNA,</t> AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.
    Rabbit Polyclonal Anti Bdnf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Representative images showing GFP and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, AAV-DIO-BDNF-mCherry+sgRNA, AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

    Journal: bioRxiv

    Article Title: Identification of a neural basis for anorexia nervosa

    doi: 10.64898/2026.02.07.704578

    Figure Lengend Snippet: (A) Representative images showing GFP and c-Fos (arrows) expression patterns in the PVT, a downstream target of the VMH, in AAV-DIO-EYFP, AAV-DIO-NaChBac+sgRNA, AAV-DIO-NaChBac+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (B) Representative images showing GFP (green) and c-Fos (red, arrows) expression patterns in the PVT, a downstream target of the VMH, in SF1-Cre::ERa-Cre::BDNFflox/++EYFP, SF1-Cre::ERa-Cre::BDNFflox/++NaChBac, SF1-Cre::ERa-Cre::BDNFflox/flox+NaChBac mice. scale bar=250 um. (C) Number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus NaChBac+sgRNA. (D) number of c-Fos positive cells in the PVT region related to panel A. n=4-5 per group; one-way ANOVA; ***P<0.001 versus EYFP; ###P<0.001 versus SF1-Cre::ERa-Cre:: BDNFflox/flox+NaChBac. (E) Diagram of viral delivery (AAV-DIO-mCherry, AAV-DIO-BDNF-mCherry+sgRNA, AAV-DIO-BDNF-mCherry+sgVglut2) into the VMH of SF1-Cre::ERa-Cre mice. (F) Body weight trajectories of SF1-Cre::ERa-Cre mice after VH injection of virus. n=4 (EYFP, BDNF+sgVglut2), n=5 (BDNF+sgRNA); two-way ANOVA; *P<0.05, **P<0.01, ***P<0.001 versus mCherry; #P<0.05 versus BDNF+sgRNA. (G) Daily food intake. n=4-5 per group, one-way ANOVA, **P=0.006 versus mCherry; #P=0.043 versus BDNF+sgRNA. (H) Total number of jumps during a 10-min test. n=4-5 per group; one-way ANOVA; ***P<0.01 versus mCherry; ###P<0.001 versus BDNF+sgRNA. (I, J) time spent in the light room in light-dark test (I), social time with stranger in three-chamber test (J). n=4-5 per group; one-way ANOVA; *P<0.05, ***P<0.01 versus mCherry; #P<0.05 versus BDNF+sgRNA. (K) Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression patterns in the PVT, a downstream of VMH, in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice. scale bar=250 um. (L) number of c-Fos positive cells in PVT region related to panel K. n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

    Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

    Techniques: Expressing, Injection, Virus

    Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression pattern in lateral septum (A), preoptic area (C), PAG (E), periLC (G). scale bar=250 μm. The pictures in the bottom row of each panel represent the magnified pictures shown in the boxed area in each column. Number of cFos positive cells in in lateral septum (B), preoptic area (D), PAG (F, periLC (H). n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

    Journal: bioRxiv

    Article Title: Identification of a neural basis for anorexia nervosa

    doi: 10.64898/2026.02.07.704578

    Figure Lengend Snippet: Representative images showing mCherry (green) and c-Fos (magenta, arrows) expression pattern in lateral septum (A), preoptic area (C), PAG (E), periLC (G). scale bar=250 μm. The pictures in the bottom row of each panel represent the magnified pictures shown in the boxed area in each column. Number of cFos positive cells in in lateral septum (B), preoptic area (D), PAG (F, periLC (H). n=4-5 per group; one-way ANOVA; ***P<0.001 versus mCherry; ###P<0.001 versus BDNF+sgRNA.

    Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

    Techniques: Expressing

    Representative images showing mCherry (red) and cFos (cyan, arrows) expression pattern in VH in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice (related to ). Scale bar=200 μm.

    Journal: bioRxiv

    Article Title: Identification of a neural basis for anorexia nervosa

    doi: 10.64898/2026.02.07.704578

    Figure Lengend Snippet: Representative images showing mCherry (red) and cFos (cyan, arrows) expression pattern in VH in mCherry, BDNF+sgRNA, BDNF+sgVglut2 injection SF1-Cre::ERa-Cre mice (related to ). Scale bar=200 μm.

    Article Snippet: The following primary antibodies were used: rabbit anti-cFos (1:1000, #2250, Cell Signaling Technology), rabbit anti-BDNF(1:200, ANT-010, Alomone Labs), goat anti-GFP (1:1000, 51370, ROCKLAND), rabbit anti-ER alpha (1:500, NBP1-84827), chicken anti-mCherry (1:500, MCHERRY-0020, aves labs).The next day, sections were rinsed three times in PBS with 0.1% Triton X-100 and incubated with secondary antibodies prepared in the same diluent (secondary antibody, 5% normal donkey serum, 0.1% Triton X-100 in PBS).

    Techniques: Expressing, Injection